Comparison of four hippurate hydrolysis methods for identification of thermophilic Campylobacter spp

Hippurate hydrolysis by Campylobacter fetus.

An additional method for differentiating between Campylobacter fetus subsp. jejuni and C. fetus subsp. intestinalis is reported. Strains of C. fetus subsp. jejuni (18/20) were shown to hydrolyze hippurate in the 2-h rapid test, whereas strains of C. fetus subsp. intestinalis did not.

Evaluation of a disk method for detection of hippurate hydrolysis by Campylobacter spp.

A disk method for detecting hippurate hydrolysis by Campylobacter spp. was evaluated and compared with the conventional tube test used at the Centers for Disease Control (CDC) (G.K. Morris and C.M. Patton, p. 302-308, in E.H. Lennette, A. Balows, W.J. Hausler, Jr., and H.J. Shadomy, ed., Manual of Clinical Microbiology, 4th ed., 1985) and high-performance liquid chromatography (HPLC). A total o...

Hippurate hydrolysis by and triphenyltetrazolium tolerance of Campylobacter fetus.

A rapid test of hippurate hydrolysis and a test of tolerance to triphenyltetrazolium chloride (TTC) were studied in 315 strains of Campylobacter fetus subsp. jejuni to determine their usefulness for biotyping this organism and for distinguishing it from C. fetus subsp. intestinalis. Of the 315 strains tested, 84% hydrolyzed hippurate and 97% were resistant to TTC. Ability to hydrolyze hippurate...

Economic cultivation of "thermophilic" Campylobacter spp.

Comparison of two methods for serotyping Campylobacter spp.

Two serotyping schemes (Penner and Laboratory of Enteric Pathogens [LEP]) based on soluble heat-stable antigens were used to analyze 3,788 Campylobacter sp. isolates. A significant percentage (36.6%) was untypeable using LEP serotyping; greater cross-reaction was also observed. The relative discrimination capabilities of the techniques were similar. Penner serotyping fulfils more of the requisi...